Author:
Su Rui,Gong Gao,Zhang Lingtian,Yan Xiaochun,Wang Fenghong,Zhang Lei,Qiao Xian,Li Xiaokai,Li Jinquan
Abstract
Abstract. Inner Mongolian Cashmere goat is an excellent local breed
selected for the dual-purpose of cashmere and meat. There are three lines of Inner
Mongolian Cashmere goat: Erlangshan, Alashan and Aerbasi. Cashmere is a kind
of precious textile raw material with a high price. Cashmere is derived from
secondary hair follicle (SHF), while hair is derived from primary hair
follicle (PHF). The growth cycle of SHF of cashmere goat is 1 year, and it
can be divided into three different stages: anagen, catagen and telogen. In
this study, we tried to find some important influence factors of SHF growth
cycle in skin tissue from Inner Mongolian Cashmere goats by RNA sequencing
(RNA-Seq). Three female Aerbasi Inner Mongolian Cashmere goats (2 years old)
were used as experimental samples in this study. Skin samples were collected
in September (anagen), December (catagen) and March (telogen) at dorsal side
from cashmere goats. Results showed that over 511 396 044 raw reads and
487 729 890 clean reads were obtained from sequence data. In total, 51
different expression genes (DEGs) including 29 downregulated genes and 22 upregulated genes were enriched in anagen–catagen comparing group. The 443 DEGs
contained 117 downregulated genes and 326 upregulated genes that were enriched
in catagen–telogen comparing group. In telogen–anagen comparing group, 779
DEGs were enriched including 582 downregulated genes and 197 upregulated
genes. The result of gene ontology (GO) annotation showed that DEGs are in
different growth cycle periods, and enriched GO items are mostly related to the
transformation of cell and protein. The Kyoto Encyclopedia of Genes and Genomes
(KEGG) enrichment result indicated that metabolic process has a great impact on
SHF growth cycle. Based on the results of a comprehensive analysis of
differentially expressed genes, GO enrichment and KEGG enrichment, we found
that FGF5, FGFR1 and RRAS had an effect on the hair follicle growth cycle. The results of
this study may provide a theoretical basis for further research on the
growth and development of SHF in Inner Mongolian Cashmere goats.
Funder
National Natural Science Foundation of China
Cited by
14 articles.
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