The effect of short/branched chain acyl-coenzyme A dehydrogenase gene on triglyceride synthesis of bovine mammary epithelial cells
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Published:2018-03-02
Issue:1
Volume:61
Page:115-122
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ISSN:2363-9822
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Container-title:Archives Animal Breeding
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language:en
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Short-container-title:Arch. Anim. Breed.
Author:
Jiang Ping, Fang Xibi, Zhao Zhihui, Yu Xianzhong, Sun Boxing, Yu Haibin, Yang RunjunORCID
Abstract
Abstract. Short/branched chain acyl-CoA dehydrogenase (ACADSB) is a member of
the acyl-CoA dehydrogenase family of enzymes that catalyze the
dehydrogenation of acyl-CoA derivatives in the metabolism of fatty
acids. Our previous transcriptome analysis in dairy cattle showed
that ACADSB was differentially expressed and was associated with milk
fat metabolism. The aim of this study was to elucidate the
background of this differential expression and to evaluate the role
of ACADSB as a candidate for fat metabolism in dairy cattle. After
analysis of ACADSB mRNA abundance by qRT-PCR and Western blot,
overexpression and RNA interference (RNAi) vectors of ACADSB gene
were constructed and then transfected into bovine mammary
epithelial cells (bMECs) to examine the effects of ACADSB on
milk fat synthesis. The results showed that the ACADSB was
differentially expressed in mammary tissue of low and high milk fat
dairy cattle. Overexpression of ACADSB gene could significantly
increase the level of intracellular triglyceride (TG), while ACADSB
gene knockdown could significantly reduce the TG synthesis
in bMECs. This study suggested that the ACADSB was important in
TG synthesis in bMECs, and it could be a candidate gene
to regulate the metabolism of milk fat in dairy cattle.
Funder
National Natural Science Foundation of China
Publisher
Copernicus GmbH
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