Technical note: An autonomous flow-through salinity and temperature perturbation mesocosm system for multi-stressor experiments
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Published:2024-01-17
Issue:1
Volume:21
Page:315-333
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ISSN:1726-4189
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Container-title:Biogeosciences
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language:en
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Short-container-title:Biogeosciences
Author:
Miller Cale A.ORCID, Urrutti Pierre, Gattuso Jean-PierreORCID, Comeau Steeve, Lebrun Anaïs, Alliouane Samir, Schlegel Robert W.ORCID, Gazeau FrédéricORCID
Abstract
Abstract. The rapid environmental changes in aquatic systems as a result of anthropogenic forcings are creating a multitude of challenging conditions for organisms and communities. The need to better understand the interaction of environmental stressors now, and in the future, is fundamental to determining the response of ecosystems to these perturbations. This work describes an automated ex situ mesocosm perturbation system that can manipulate several variables of aquatic media in a controlled setting. This perturbation system was deployed in Kongsfjorden (Svalbard); within this system, ambient water from the fjord was heated and mixed with freshwater in a multifactorial design to investigate the response of mixed-kelp communities in mesocosms to projected future Arctic conditions. The system employed an automated dynamic offset scenario in which a nominal temperature increase was programmed as a set value above real-time ambient conditions in order to simulate future warming. A freshening component was applied in a similar manner: a decrease in salinity was coupled to track the temperature offset based on a temperature–salinity relationship in the fjord. The system functioned as an automated mixing manifold that adjusted flow rates of warmed and chilled ambient seawater, with unmanipulated ambient seawater and freshwater delivered as a single source of mixed media to individual mesocosms. These conditions were maintained via continuously measured temperature and salinity in 12 mesocosms (1 control and 3 treatments, all in triplicate) for 54 d. System regulation was robust, as median deviations from nominal conditions were < 0.15 for both temperature (∘C) and salinity across the three replicates per treatment. Regulation further improved during a second deployment that mimicked three marine heat wave scenarios in which a dynamic temperature regulation held median deviations to < 0.036 ∘C from the nominal value for all treatment conditions and replicates. This perturbation system has the potential to be implemented across a wide range of conditions to test single or multi-stressor drivers (e.g., increased temperature, freshening, and high CO2) while maintaining natural variability. The automated and independent control for each experimental unit (if desired) provides a large breadth of versatility with respect to experimental design.
Funder
European Commission
Publisher
Copernicus GmbH
Subject
Earth-Surface Processes,Ecology, Evolution, Behavior and Systematics
Reference30 articles.
1. Bass, A., Wernberg, T., Thomsen, M., and Smale, D.: Another Decade of Marine Climate Change Experiments: Trends, Progress and Knowledge Gaps, Front. Mar. Sci., 8, ISSN: 2296-7745, 2021. 2. Bhatia, M. P., Kujawinski, E. B., Das, S. B., Breier, C. F., Henderson, P. B., and Charette, M. A.: Greenland meltwater as a significant and potentially bioavailable source of iron to the ocean, Nat. Geosci., 6, 274–278, https://doi.org/10.1038/ngeo1746, 2013. 3. Bindoff, N. L., Cheung, W. W. L., Kairo, J. G., Arístegui, J., Guinder, V. A., Hallberg, R., Hilmi, N., Jiao, N., Karim, M. S., Levin, L., O'Donoghue, S., Purca Cuicapusa, S. R., Rinkevich, B., Suga, T., Tagliabue, A., and Williamson, P.: Changing Ocean, Marine Ecosystems, and Dependent Communities, in: IPCC Special Report on the Ocean and Cryosphere in a Changing Climate, edited by: Pörtner, H.-O., Roberts, D. C., Masson-Delmotte, V., Zhai, P., Tignor, M., Poloczanska, E., Mintenbeck, K., Alegría, A., Nicolai, M., Okem, A., Petzold, J., Rama, B., and Weyer, N. M., Cambridge University Press, Cambridge, UK and New York, NY, USA, 447–587, https://doi.org/10.1017/9781009157964.007, 2019. 4. Blois, J. L., Williams, J. W., Fitzpatrick, M. C., Jackson, S. T., and Ferrier, S.: Space can substitute for time in predicting climate-change effects on biodiversity, P. Natl. Acad. Sci. USA, 110, 9374–9379, https://doi.org/10.1073/pnas.1220228110, 2013. 5. Divya, D. T. and Krishnan, K. P.: Recent variability in the Atlantic water intrusion and water masses in Kongsfjorden, an Arctic fjord, Polar Sci., 11, 30–41, https://doi.org/10.1016/j.polar.2016.11.004, 2017.
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