Identification of genomic regions and candidate genes of functional importance for gastrointestinal parasite resistance traits in Djallonké sheep of Burkina Faso
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Published:2019-06-05
Issue:1
Volume:62
Page:313-323
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ISSN:2363-9822
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Container-title:Archives Animal Breeding
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language:en
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Short-container-title:Arch. Anim. Breed.
Author:
Álvarez Isabel, Fernández Iván, Soudré Albert, Traoré Amadou, Pérez-Pardal Lucía, Sanou Moumouni, Tapsoba Stephane A. R., Menéndez-Arias Nuria A., Goyache FélixORCID
Abstract
Abstract. A total of 184 Djallonké lambs from Burkina Faso with phenotypes
for packed-cell volume (PCV), log-transformed fecal egg count (lnFEC), and
FAffa MAlan CHArt (FAMACHA©) eye scores were typed with the OvineSNP50 BeadChip of Illumina to contribute to the knowledge of the genetic basis of
gastrointestinal (GIN) parasite resistance in sheep. Association analysis
identified a total of 22 single-nucleotide polymorphisms (SNPs) related with PCV (6 SNPs), lnFEC (7), and FAMACHA
scores (9) distributed among 14 Ovis aries chromosomes (OAR). The identified SNPs
accounted for 18.76 % of the phenotypic variance for PCV, 21.24 % for
lnFEC, and 34.38 % for FAMACHA scores. Analyses pointed out the importance
of OAR2 for PCV, OAR3 for FAMACHA scores, and OAR6 for lnFEC. The 125 kb
regions surrounding the identified SNPs overlapped with seven previously
reported quantitative trait loci (QTLs) for the traits analyzed in the current work. The only
chromosome harboring markers associated with the three traits studied was
OAR2. In agreement with the literature, two different chromosomal areas on
OAR2 can play a major role in the traits studied. Gene-annotation enrichment
analysis allowed us to identify a total of 34 potential candidate genes for PCV
(6 genes), lnFEC (4), and FAMACHA scores (24). Annotation analysis allowed us to
identify one functional term cluster with a significant enrichment score
(1.302). The cluster included five genes (TRIB3, CDK4, CSNK2A1, MARK1, and SPATA5) involved in
immunity-related and cell-proliferation processes. Furthermore, this
research suggests that the MBL2 gene can underlie a previously reported QTL for
immunoglobulin A levels on OAR22 and confirms the importance of genes
involved in growth and size (such as the ADAMTS17 gene on OAR18) for GIN resistance
traits. Since association studies for the ascertainment of the genetic basis
of GIN resistance may be affected by genotype–environment interactions,
obtaining information from local sheep populations managed in harsh
environments contributes to the identification of novel genomic areas of
functional importance for GIN resistance for that trait.
Funder
International Foundation for Science
Publisher
Copernicus GmbH
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