Detection of one large insertion/deletion (indel) and two novel SNPs within the <i>SPEF2</i> gene and their associations with male piglet reproduction traits
-
Published:2016-06-15
Issue:2
Volume:59
Page:275-283
-
ISSN:2363-9822
-
Container-title:Archives Animal Breeding
-
language:en
-
Short-container-title:Arch. Anim. Breed.
Author:
Chen Rui,Yu Shuai,Ren Fa,Lv Xiao Yan,Pan Chuan Ying
Abstract
Abstract. The sperm flagella 2 (SPEF2) gene is essential for normal sperm tail development and male fertility. To fully characterize the structure of the mutation and to further study the function of the pig SPEF2 gene, we explored the insertion/deletion (indel) and novel single-nucleotide polymorphisms (SNPs) within the pig SPEF2 gene, and tested their associations with the testicular sizes in male Large White (LW) and Landrace (LD) pigs from China. Herein, a large insertion located at the SPEF2 gene in chromosome 16 was found, and two alleles of "I" (insertion) and "D" (deletion) were designated. Allele "D" was dominant in all analyzed pigs. Two novel SNPs (namely (NC_010458) g.19642G > A, resulting in AfaI aCRS PCR–PFLP, and g.19886C > G, resulting in EcoRI aCRS PCR–PFLP) were found in LW and LD pigs. Association testing revealed that g.19886C > G was significantly associated with the testis long circumference (TLC) in LW pigs (P < 0.05), suggesting that this SNP would be the DNA marker for the marker-assisted selection (MAS) in reproduction traits. This preliminary result indicates that the pig SPEF2 gene had significant effects on male reproduction traits. These findings could not only extend the spectrum of genetic variations in the pig SPEF2 gene but also contribute to implementing MAS in genetics and breeding in pigs.
Funder
China Postdoctoral Science Foundation
Publisher
Copernicus GmbH
Reference30 articles.
1. Andersson, M., Peltoniemi, O. A. T., Makinen, A., Sukura, A., and Rodriguez- Martinez, H.: The hereditary “short tail” sperm defect – a new reproductive problem in Yorkshire Boars, Reprod. Domest. Anim., 35, 59–63, 2000. 2. Beerda, B., Wyszynska-Koko, J., Te Pas, M. F., de Wit, A. A., and Veerkamp, R. F.: Expression profiles of genes regulating dairy cow fertility: recent findings, ongoing activities and future possibilities, Animal, 2, 1158–1167, 2008. 3. Bergfelder-Drüing, S., Grosse-Brinkhaus, C., Lind, B., Erbe, M., Schellander, K., Simianer, H., and Tholen, E.: A genome-wide association study in large white and landrace pig populations for number piglets born alive, PLoS One, 10, e0117468, https://doi.org/10.1371/journal.pone.0117468, 2015. 4. Chen, F., Shi, J., Luo, Y. Q., Sun, S. Y., and Pu, M.: Genetic Characterization of the Gypsy Moth from China (Lepidoptera, Lymantriidae) Using Inter Simple Sequence Repeats Markers, PLoS One, 8, e73017, https://doi.org/10.1371/journal.pone.0073017, 2013. 5. Devkota, B., Koseki, T., Matsui, M., Sasaki, M., Kaneko, E., Miyamoto, A., Amaya, Montoya, C., and Miyake, Y.: Relationships among age, body weight, scrotal circumference, semen quality and peripheral testosterone and estradiol concentrations in pubertal and postpubertal Holstein bulls, J. Vet. Med. Sci., 70, 119–121, 2008.
Cited by
14 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|