Novel alternative splicing variants of <i>ACOX1</i> and their differential expression patterns in goats
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Published:2018-01-24
Issue:1
Volume:61
Page:59-70
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ISSN:2363-9822
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Container-title:Archives Animal Breeding
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language:en
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Short-container-title:Arch. Anim. Breed.
Author:
Wu Xian-Feng,Liu Yuan,Gao Cheng-Fang,Chen Xin-Zhu,Zhang Xiao-Pei,Li Wen-Yang
Abstract
Abstract. As the first and rate-limiting enzyme of the peroxisomal β-oxidation
pathway, acyl-coenzyme A oxidase 1 (ACOX1), which is regulated by peroxisome
proliferator-activated alfa (PPARα), is vital for fatty acid
oxidation and deposition, especially in the lipid metabolism of very
long-chain fatty acids. Alternative splicing events of ACOX1 have been
detected in rodents, Nile tilapia, zebra fish and humans but not in goats.
Herein, we identified a novel splice variant of the ACOX1 gene,
which was designated as ACOX1-SV1, in addition to the complete transcript,
ACOX1, in goats. The length of the ACOX1-SV1 coding sequence was 1983 bp,
which presented a novel exon 2 variation owing to alternative 5′-splice
site selection in exon 2 and partial intron 1, compared to that in ACOX1. The
protein sequence analysis indicated that ACOX1-SV1 was conserved across
different species. Reverse-transcription quantitative real-time polymerase
chain reaction (RT-qPCR) analysis showed that these two isoforms were
expressed spatially and differently in different tissue types. ACOX1 and
ACOX1-SV1 were expressed at high levels in liver, spleen, brain and adipose
tissue in kid goats, and they were abundantly expressed in the fat, liver and
spleen of adults. Interestingly, whether in kids or in adults, in fat, the
mRNA level of ACOX1 was considerably higher than that of ACOX1-SV1. In
contrast, in the liver, the expression of ACOX1-SV1 was considerably higher
than that of ACOX1. This differential expression patterns showed the
existence of a tissue-dependent splice regulation. These novel findings for
ACOX1 should provide new insights for further studies on the function of
ACOX1 and its variants that should aid in the breeding of goats with improved
meat quality.
Publisher
Copernicus GmbH
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