TIMP1 may affect goat prolificacy by regulating biological function of granulosa cells-Reference-Cited by-同舟云学术

TIMP1 may affect goat prolificacy by regulating biological function of granulosa cells

Published:2022-03-10 Issue:1 Volume:65 Page:105-111
ISSN:2363-9822
Container-title:Archives Animal Breeding
language:en
Short-container-title:Arch. Anim. Breed.

Author:

Hong Lei^ORCID,Chen Xiang,Zhu Min,Ao Zheng,Tang Wen,Zhou Zhinan

Abstract

Abstract. Tissue inhibitor of metalloproteinase 1 (TIMP1) is associated with animal reproductive processes, such as follicular growth, ovulation, luteinization, and embryo development in mammals. The purposes of this study were to explore the expression and localization of TIMP1 in the ovarian tissues and determine the effect of TIMP1 on the function of granulosa cells and the association of TIMP1 with lambing-related genes of the goats. Immunohistochemical analysis showed that TIMP1 protein was strongly expressed by granulosa cells. Enzyme-linked immunosorbent assay (ELISA) results showed that TIMP1 overexpression promoted the secretion of estradiol of granulosa cells after 12, 24, and 48 h of transfection. Moreover, in vitro experiments indicated that TIMP1 had the ability to promote the cell proliferation and elevate the transcriptional levels of four genes associated with goat prolificacy, including BMPR-1B, BMP15, GDF9, and FSHB, in granulosa cells. In conclusion, TIMP1 could be an important molecule in regulating reproductive performance of the goats by affecting estrogen secretion and cell proliferation, as well as the expression of lambing-related genes of granulosa cells in the goats.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Publisher

Copernicus GmbH

Link

https://aab.copernicus.org/articles/65/105/2022/aab-65-105-2022.pdf

Reference37 articles.

1. An, X. P., Hou, J. X., Li, G., Peng, J. Y., Liu, X. Q., Liu, H. Y., Xiao, W. P., Wang, J. G., Song, Y. X., and Cao, B. Y.: Analysis of Differentially Expressed Genes in Ovaries of Polytocous versus Monotocous Dairy Goats Using Suppressive Subtractive Hybridization, Repord. Domest. Anim., 47, 498–503, 2012.

2. Anchamparuthy, V. M., Dhali, A., Lott, W. M., Pearson, R. E., and Gwazdauskas, F. C.: Vitrification of bovine oocytes: implications of follicular size and sire on the rates of embryonic development, J. Assist. Repord. Gen., 26, 613–619, 2009.

3. Arpino, V., Brock, M., and Gill, S. E.: The role of TIMPs in regulation of extracellular matrix proteolysis, Matrix Biol., 44–46, 247–254, 2015.

4. Cui, H. X., Zhao, S. M., Cheng, M. L., Guo, L., Ye, R. Q., Liu, W. Q., and Gao, S. Z.: Cloning and Expression Levels of Genes Relating to the Ovulation Rate of the Yunling Black Goat, Biol. Reprod., 80, 219–226, 2009.