Induction of direct organogenesis in Blackberry (Rubus fruticosusL.) using leaf explants

Abstract

This study is to establish the best conditions for in vitro propagation by inducing direct organogenesis of “Thornfree” blackberry (Rubus fruticosusL.) using different doses of plant growth regulators, which affect the regeneration of shoots in compound nutrient medium of mineral salts Murashige and Skoog (MS). Young leaves were used as explants. In the initiation phase, plant material necessary for the experiment was obtained, using an MS nutrient medium enriched with 0.5 mg. l-1of BAP, after 30 days’ leaves were obtained that were introduced in regeneration medium where the effect of BAP (1 and 2 mg. l-1) was evaluated in combination with AIA (0.5 mg. l-1) and TDZ (0.5 mg. l-1). The highest number of shoots with an average of 6.40 and oxidation percentage of 7.41% was achieved. In the proliferation phase no significant statistical differences were observed, however, the optimum performance attained for the number of buds was obtained with a concentration of 2 mg. l-1of BAP, having an average shoot-length of 6.10 cm and a rooting percentage of over 80%.