Comprehensive analysis of the analytical characteristics and structure of the lateral branches pectin isolated from seeds of wild medlar (Mespilus germanica l.)

Abstract

The medlar (Mepilus germanica L.) is an important element of the flora of the mountainous part of the Guba district, and its biotechnological potential has been little studied. This study was conducted in order to comprehensively analyze the analytical characteristics of pectin isolated from the seeds of the fruits of this plant. In the mode of low frequency hydroacoustic cavitation, primary extracts of fresh seeds of fruits of technical maturity were obtained, which were then purified according to a multi-stage scheme using a filtering centrifuge, a diatomite filter, a decanter and ultra- and diafiltration and subjected to low-temperature concentration and spray drying. The analysis of the studied pectin using titrometric, spectrometric and chromatographic conventional methods showed that the pectin of wild medlar seeds is a mixture of linear and branched polymers of a-D-galacturonane and other polysaccharides (mainly high molecular weight). It is pectin with a high degree of esterification (72.4 ± 0.6%) and a high content of the methoxyl component (48±0.25% of acetyl groups in the total mass of galacturonic acids). At the same time, the content of free carboxyl groups in it is quite low and amounted to 4.25 ± 0.09%, which indicates its low complexing ability.