Indigenous Bacteria Diversity of Fermetodege Based on Molecular Analysis The Next Generation Sequencing (NGS)

Abstract

Fermetodege was fermented feed that was developed by researchers which is proven to increase the biomass and semen quality of ruminant livestock. The raw material for making this fermented feed were cellulose-rich materials, so this fermented feed contains many kinds of cellulolytic bacteria.The objective of this study was to reveal the diversity of indigenous bacteria in a fermented feed is called Fermetodege. Fermetodege is made from fermented water hyacinth (Eichhornia crassipes), corn (Zea mays) cobs and rice (Oryza sativa) bran. This mixture is rich in cellulose and a good source of cellulolitic bacterial isolates. The cellulolitic bacteria is source of cellulase enzyme. Revealing of bacterial diversity is carried out through a series of procedures which include making a fermetodege, fermenting it and taking samples in the mesophilic I, thermophilic, mesophilic II and maturing phase. The bacterial community in the feed was dominated by groups (from the most to the least), namely Bacilli, Clostridia, Gammaproteobacteria, Bacteroidia, Negativicutes, Actinobacteria and Desulvovibrionia, Alfaproteobacteria and Coriobacteria. Based on the NGS results, it was found that there were 9 groups of bacterial communities in the fermented feed.