Author:
Krisdahasti Lenggang Mamitu,Hardaningsih Ignatius,Sari Dini Wahyu Kartika
Abstract
This study was designed to evaluate a DMSO (Dimethyl Sulfoxide) and sucrose combination as a cryoprotectant for cryopreservation of Najawa carp (Cyprinus carpio L.) sperm. The experiment was conducted according to the Completely Randomized Design (CRD) with four treatments 10% DMSO + 0.2 M sucrose, 10% DMSO + 0.1 M sucrose, 5% DMSO + 0.2 M sucrose, 5% DMSO + 0.1 M sucrose. Each treatment consisted of three replications. The parameter observed were sperm progressive motility of fresh sperm, diluted sperm before freezing, and diluted sperm after freezing 1 day; 7 days; 14 days; 21 days; 28 days. The data were analyzed by ANOVA. The result showed that there was no significant difference between treatments (Sig. > 0.05). The highest relative protection was 89.51% carried out by 5% DMSO + 0.1 M sucrose.
Reference14 articles.
1. KARAKTER FENOTIPE DAN GENOTIPE IKAN MAS “MERAH MENYALA” NAJAWA DARI CANGKRINGAN, JOGJAKARTA SERTA POTENSI EKONOMISNYA
2. Gwo J.C.. Cryopreservation of Sperm of Some Marine Fishes, in Tiersch T.R. and Mazik P.M. (Eds.), Cryopreservation in Aquatic Species. World Aquaculture Society, Baton Rouge, L.A. 138-160 (2000)
3. Chew P.C. and Zulkafli Abd. R.. Sperm Cryopreservation of Some Freshwater Fish Species in Malaysia. Agricultural and Biological Sciences: “Current Frontiers in Cryopreservation” (2012)
4. Effect of different avian egg yolk types on fertilization ability of cryopreserved common carp (Cyprinus carpio) spermatozoa