Determination of protodioscin in tribulus terrestris by reversed-phase high-performance liquid chromatography

Abstract

In this study, high performance liquid chromatography (HPLC) was used to analyze the protodioscin content in Tribulus terrestris samples. They were separated by C18 chromatography column, gradient with 0.1 % phosphoric acid solution and acetonitrile was used as the mobile phase with the flow rate of 0.5 mL/min, the column temperature was 45 °C, and the detection wavelength was 203 nm. The results showed that the protodioscin content has a good linear correlation (r>0.999) within the range of 50 mg/L to 1500 mg/L. In samples of raw material from Crimea it was found: 0.546 % ± 0.013 protodioscin and in analyzed material from Western Siberia it was 0.338% ± 0.008 for n=3. Raw materials of Tribulus terrestris growing in Crimea are more promising than similar raw materials from the West Siberian region due to the higher content of protodioscin for the production of biologically active products containing steroid saponins.