Inhibitory effect of plant metabolites of Nigella sativa on conditionally pathogenic microflora of productive animals

Abstract

Objective: The effectiveness of a phytobiotic based on active metabolites of Nigella sativa L. against typical microorganisms of opportunistic microbiocenosis of the mucous membranes of highly productive cows was studied. Initially, the inhibitory activity of the phytobiotic was studied on cultures of wild multi-antibiotic-resistant isolates of P. aeruginosa and S. aureus isolated on a commercial dairy farm. It was found that the phytobiotic had the ability to inhibit the growth of isolates on the nutrient medium, but the severity of the inhibitory effect varied notably. At the second stage, an experiment was conducted with the local application of phytobiotics on cows that had inflammatory complications of the postpartum period. The results of the experiment showed a pronounced inhibitory effect of the phytobiotic on S. aureus, Str. uberis, P. aeruginosa, E. cloacae, C. albicans, and P. mirabilis. There was an involution of inflammatory symptoms and normalization of the clinical and microbiological state of the mucous membrane after the use of a phytobiotic preparation.