Study of the penetrating ability of 5% glucose solution into insulin–dependent cells of mouse organs, under the influence of ionising radiation

Abstract

The aim of the work is to study the penetrating ability of a 5% glucose solution into damaged insulin-dependent tissues of the body, under the influence of various doses of ionising radiation. Hypothesis. Since there is a certain level of glucose in the blood, it is possible to assume increased damage to cells under the influence of an excessive amount of glucose penetrating into cells not only with the help of carrier proteins, but also through radiation-damaged cell membranes. Materials and methods. In order to assess the damaging effect of ionising radiation, insulin-dependent organs were used. The animals were divided into 3 groups. The first group consisted of organs of 10 mice that received a dose of ionising radiation of 1 g. The second group consisted of organs of 10 mice that received a dose of ionising radiation of 2 Gy. The third group consisted of the organs of 10 mice that received a dose of 3Gн ionising radiation. Results and discussion. Depending on the dose of irradiation of organ groups, we found a direct dependence of the decrease in the content of residual glucose in the nutrient medium 6 hours after irradiation. And inversely proportional dependence of glucose content 24 hours after irradiation. The strongest penetrating power of 5% glucose is observed when irradiated with a radiation dose of 2 Gy. Under the influence of ionising radiation, target cells were stained more intensively with hematoxylin-eosin than intact tissues, regardless of the radiation dose. Conclusions. Therefore, the in vitro study reflects both changes in residual glucose level in the nutrient medium, without active participation of glucose and insulin carrier proteins, and intracellular changes under the influence of ionising radiation damaging factors on animal organ cells.