Simulation of choroidal neovascularization in rabbits using indirect transpupillary laser coagulation

Abstract

Objective: to identify the applicability of indirect transpupillary laser coagulation (ITLC) for modeling choroidal neovascularization (CN) in rabbits and its optimal parameters. Materials and methods: 12 rabbits, divided into 2 equal groups, were subjected to single- and double-sided ITLC, respectively, using a laser device with a wavelength of 810 nm (using different powers), an indirect ophthalmoscope and a lens. The time spent on coagulation, ophthalmoscopic signs on the 1st, 8th, 15th, 22nd and 29th days of the study, the severity of CN (histological and immunohistochemical (IHC) studies), body weight and general condition of the animals were also evaluated. Results: in all cases, it was possible to apply the coagulants using ITLC. During ophthalmoscopy, peripheral vascularization was detected on the 8th-29th day. Histological and IHC studies revealed significant tissue damage at a power of 700, 1000 mW, as well as signs of CN when using a power of 500 mW on the 15th day of the study. Conclusion: ITLC is a safe method and can be used to simulate CN in rabbits at a power of no more than 500 mW.