Use of SSR markers to study genetic polymorphism in members of the Ribes L. genus from the VNIISPK collection

Abstract

This study was the first time in Russia to carry out a large-scale (127 samples) assessment of the diverse gene pool of black currant and red currant, the study of intervarietal polymorphism between varieties of the Ribes L. genus from the VNIISPK collection. A cluster analysis of the genetic similarity of black currant varieties (53 varieties), red currant (73 varieties) and one gooseberry variety was performed using 14 SSR markers. Based on the data obtained, a dendrogram was built, in which a number of clusters with high bootstrap support (BS, %) were distinguished. At BS>50%, the coefficients of pairwise genetic similarity between varieties varied from 0.4 to 0.91. The studied representatives of the Ribes genus merged into two main clusters of red currant and black currant. The blackcurrant cluster was joined by gooseberries. The SSR analysis method allows to reveal broad perspectives in the field of identifying varieties affiliation to species.