Some aspects of clonal micropropagation of Amelanchier Medik. genus representatives

Abstract

The article presents studies of organogenesis peculiarities of varieties belonging to various species of Amelanchier Medik. genus in in vitro culture at the stages of micropropagation, rooting and depositing. Mineral composition and growth regulator for active adventive microshoots formation were determined. The optimal auxin source for rhizogenesis of various Amelanchier alnifolia varieties has been established. Conditions for explants' preservation in aseptic conditions for 12 months have been revealed. Different effects of cytokinin type substances on shadberry regeneration potential under aseptic conditions were noted. Analysis of regenerants' morphometric indicators showed the effectiveness of using 6-benzylaminopurin (BAP) in the concentration of 1.0 mg/L for shadberry varieties' clonal micropropagation and indolebutyric acid (IBA) at 1.0 mg/L concentration for microshoots' rooting.