Antimycotic activity of bacterial strains against the pathogen of grape necrotic leaf spotting Alternaria sp.

Abstract

Species of the genera Bacillus Cohn and Pseudomonas Migula are successfully used as biocontrol agents for many mycopathogens, including Alternaria sp. To assess the bacteria antagonistic potential against Alternaria sp, we used the method of counter cultures on various nutrient media – universal and specialized. Of the 24 bacterial strains of the genus Bacillus, 18 strains were developed antagonistic activity to Alternaria sp. after 10 days of co-cultivation on the Tylona-3 medium. The maximum sterile zone (8.0 mm) was formed by bacterial strains (R-9, 5B-1, 01 cor f Bacillus sp.). 3 strains of 9 bacteria of the genus Pseudomonas, developed antagonistic activity to Alternaria sp. on Kinga B medium - 14-3 Pseudomonas sp., Oif 2-1 Pseudomonas sp., 14-4 Pseudomonas sp. The maximum antibiotic activity on potato-sucrose agar was established in strains 3-3 Bacillus sp., and K 1-1 Bacillus sp. (sterile zone 7.5 mm). All the tested bacteria most often caused one sign of antagonism – antibiosis.