Growing optimization of suspension cultures of medicinal plant cells

Author:

Kolomietc Andrey,Nicolaeva Nadezda,Larina Victoria,Chupakhina Nataliya

Abstract

Suspension cell cultures allow to save plant material when obtaining biologically active compounds of natural origin. As a result of the studies, optimal parameters were selected to increase the formation of biologically active metabolites in suspension cell cultures of such medicinal plants as Maackia amurensis Rupr., Hyssopus officinalis L. and Saposhnikovia divaricata (Turcz.) Schischk. Medicinal plants are a large group of plants used as raw materials for the production of medicinal and preventive drugs for medical and animal use. The assortment of phytopreparations is constantly expanding due to the increased demand for natural remedies, due to their less aggressive and toxic nature compared to synthetic ones [1]. Cultivation of medicinal plants in the form of isolated cells in vitro is one of the most modern technologies for rapidly obtaining a large biomass of plant material with stable growth features year-round under controlled conditions [2]. It is known that cells in vitro grow faster and have peculiarities of synthesis and accumulation of biologically active substances compared to intact plants [3]. Isolated cells, unlike tissue cells, also have an advantage for their use as a source of active metabolites, since they have the ability to release these compounds into the intercellular space [4]. The goal of this paper was to select parameters for increasing the biosynthetic activity of cultured suspension cultures of medicinal plant cells in vitro by optimizing cultivation conditions and introducing precursors of secondary metabolite biosynthesis into the nutrient media.

Publisher

EDP Sciences

Reference10 articles.

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2. Butenko R.G., Biology of Higher Plant Cells in Vitro and Biotechnology Based on Them (FBC Press, Moscow, 1999)

3. Shin K.S., J. of Microbiology, 42, 1 (2004)

4. Zagorskaya A.A., Deineko E.V., Plant Physiology J., 64, 6 (2017)

5. Murashige T., Skoog F., Physiologia plantarum J., 15, 3 (1962)

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