Effects of Putrescine and Spermine on Pollen Germination Levels of Some Olive Cultivars

Abstract

In this study, it is aimed to determine the effects of putrescine and spermine on pollen germination levels of 8 olive cultivars. Arbequina, Gemlik, Halhalı, Karamani, Kozan yerli, Saurani, Sayfi and Şami olive cultivars were used as material. In the study, 5 spermine doses (0,25mM, 0,125 mM, 0,025 mM, 0,05 mM and 0,005 mM) and 5 putrescine doses (0,20 mM, 0,10 mM, 0,05 mM, 0,025 mM and 0,1 mM) added to basal germination medium (15% sucrose + 1% agar + 100 ppm Boric acid) were tested for determining the effects on pollen germination of olive cultivars. Basal media was used for Control treatment. In terms of pollen germination media, pollen germination levels were increased with lower putrescine and higher spermine levels. In Sayfi olive cultivar, while pollen viability rate was 42,74%, pollen germination was 0,00% in control treatments. However, pollen germination was increased for Sayfi olive cultivar with 0,20 mM and 0,10 mM putrescine (3,64% and 3,40%, respectively) and 0,25 mM, 0,125 mM and 0,05 mM Spermine (12,56%, 4,73% and 3,40%, respectively). In conclusion, polyamine treatments were increased the low germinated cultivars. So, polyamine treatments reveal the potential of the pollen especially in low-germinated pollens.