The effect of serum lipids and spermoplasm on the stallion sperm quality

Abstract

The quality and cryoresistance of stallion sperm depend on many endogenous and exogenous factors. One of the factors affecting the sperm quality are the parameters of the lipid spectrum of spermoplasm and blood serum. Lipase activity, cholesterol and triglyceride content in blood serum and spermoplasm were studied in 19 stallions of the Arabian breed aged from 5 years to 15 years (on average 10.1±0.3 years). It was found that lipase activity in the spermoplasm (217.0 U/l) is on average 11.4 times higher than in the blood serum (19.1 U/l). The concentration of spermoplasm triglycerides (1.3 mmol/l) is on average 4.3 times higher than in blood serum (0.3 mmol/l). The concentration of cholesterol in the blood serum (2.4 mmol/ l) is on average 12 times higher than the same indicator in the stallion spermoplasm (0.2 mmol/l). The relationship between the parameters of the lipid spectrum of blood serum and spermoplasm with the characteristics of stallion sperm was studied. A significant relationship was found between the activity of lipase in blood serum with progressive mobility (r=0.66; p=0.006) and survival (r=0.67; p=0.005) of spermatozoa in cryopreserved sperm.