To Check Efficacy of Different Disinfectants by Kelsey Sykes Method

Abstract

The incidence of hospital and community acquired infections has globally increased worldwide. The concentration of the disinfectants is important to give its higher efficacy against pathogens or microorganisms. Microorganisms are part of or environment cause major or severe diseases to reduce the risk of these diseases we generally clean surrounding by using cleaning agents like soaps, antiseptics or disinfectants. So to check weather these disinfectants are performs there work against specific bacteria or microorganism we performed this test to check the effectiveness of disinfectants against specific microorganism and fungus. Five bacterial types that are frequently acquired in hospitals from which two are gram positive organism, two are gram negative organism and single strain of fungus ‘(Pseudomonas aeruginossa, Klebsiella pneumoniae, Staphylococcus aureus (MRSA), Acinetobacter baumannii, Candida tropicalis) (clinical isolates) were examined for their susceptibility against 3 commonly used disinfectants hospitals Chlorine releasing agent, Quaternary ammonium compound and Aldehyde free disinfectant). The turbidity in the nutrient broth and the growth in culture media showed that all three disinfectants had lowered the microbial growth of every clinical strain. In general, the reference strains have lower growth and turbidity than the clinical strains. When combined, our results demonstrated that every product we examined had a high disinfection killing rate against microorganisms from various sources, indicating the excellent caliber of these goods. In response to rising disinfectant usage, hand sanitizer production has expanded to keep up with demand. Normal flora or microorganisms are part of our body in everyday life in which some are necessary some are unnecessary and can cause major or severe diseases to avoid from these diseases we generally clean our self and surrounding by using cleaning agents like soaps, antiseptics or disinfectants. So, to check weather these sterilizing agents are performed their work against specific bacteria or microorganism we performed this test to check the effectiveness of disinfectants against specific microorganism and fungus.