Micropropagation of rootstocks of stone fruit cultures in vitro

Abstract

The usage of the method of clonal micropropagation of plants is currently the most promising for obtaining virus-free plant material, including clonal rootstocks of stone fruit cultures. The introduction of clonal rootstocks of stone fruit cultures PK SK1, PK SK 2, AI 1 into invitro culture is best doneduring the active shoot growth period in the second decadeof May. During this period, the highest level of explants regeneration was noted: in the rootstock PK SK 1 – 94,6 %, in the rootstock AI 1 78.2%, in PK SK 2 80.4%, in Gisela 5 (control) 85,7%. From the third decade of May, the survival rate of explants begins to decline. At the stage of multiplication on the Murashige-Skoog medium, with the addition of 6-BAP 1 mg/L, the average multiplication factor of clonal rootstocks to the fourth passage was 1: 12 for PK SK1, for Gisela 5 and for AI 11: 8.