Application of bioactive compounds for increasing production of grape planting materials and higher germination of hybrid seeds

Abstract

The paper presents the results of studying biological methods of increasing the yield of planting material for grapes and increasing the germination of hybrid seeds to accelerate the selection process. The impact of bioactive compounds (6-benzylaminopurine and gibberellic acid) in culture medium on production of grape clones during micropropagation stage was studied. Modified culture medium of the A.N. Rebrov’s patent was determined as having the most potential on the first stage of grape explant cultivation. The establishment of grape regenerant plants was up to 80%, depending on the variety. On the second and third passages Rebrov’s culture medium was modified by the addition of 6-benzylaminopurine in the concentration 0.35 mg/l and gibberellic acid in the concentration 0.1 mg/l. The method of prestratification soaking of seedlings seeds in the solutions of gibberellic acid of various concentrations was also studied to accelerate the breeding process. The production of seedlings increased to 91.2% at the concentration of gibberellic acid 0.01% that was higher than control production on 31.2%.