Research of the survival of a consortium of Zygosaccharomyces kombuchaensis yeast and Gluconoacetobacter xylinus bacteria during frozen storage using various protective media

Abstract

In the present study the survival of the consortium of yeast and bacteria using protective media was investigated. The protective medium consisting of 1 % gelatin, 5 % monosodium glutamate and 5 % sucrose provides the best preservation of living cells of the consortium and allows stabilization of cells for up to 100 days at storage temperatures from -2 °C to -10 °C. The projective medium consisting of 1 % gelatin and 10 % sucrose provides effective storage of the cells at t=-2 °C for 100 days; t=-5 °C – 80 days; t=-10 °C – 60 days. The projective medium of skimmed milk and 7.5 % glucose ensures effective storage of the cells of the consortium at t=-2 °C for 100 days; t=-5 °C – 80 days; t=-10 °C – 80 days. The information obtained is interesting for following researches of the development of technologies for the bioconversion of plant materials.