High Frequencies Repetitive Shoots organogenesis of Potato (Solanum Tuberosum L.) Under Partial Immersion Bioreactor Culture

Author:

Agisimanto Dita,Devy Nirmala Friyanti,Yulianti Farida,Hardiyanto ,Hadiba Nur Hayati

Abstract

Potato plays important role in nutritional security, poverty alleviation, and income generation in Indonesia. Potato production restricted and inconsistent by lower quality of seedlings application. Solid medium technique for long time has been using for producing pathogen free plants. This procedure has typically costly as labor intensive and longer durability of production. Liquid culture medium allows the close contact to explants which stimulates and facilitates the nutrients and hormones uptake leading to higher rate multiplication and shoot and root growth of higher quality seedling mother plants. The aim of study is to introduce partial immerse bioreactor system (PIB) for high frequencies of granola Kembang (GK) organogenesis and survival rate during weaning stage in nursery. A 20 shoots of GK were transferred to PIB containing MS medium aerated with 0.5, 1.0 and 1.5 vvm air atmosphere during 30 days of culture. High rate repetitive shoots organogenesis were observed from PIB culture, significantly different to semisolid culture. Averagely 500, 616 and 602 new shoots were harvested from 0.5, 1.0 and 1.5 vvm treatment respectively, which are 30, 37 and 36 folds compared to semisolid culture. Approximately 95-98% of regenerated shoots were survived in acclimatization chamber for both PIB and semisolid culture.

Publisher

EDP Sciences

Subject

General Medicine

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3