Abstract
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) gene editing system plays a role in the inhibition of immunity in many bacteria and archaea equips with various advantages such as high efficiency, diversity, and modularity. It is now widely used to improve quality and quantity of crops to satisfy global food demand. Although these prospects are tempting, deeper understanding is still required to improve their efficiency and safety. Therefore, an overview of this special system is important. In this review, the current knowledge of different types of CRISPR/Cas system as well as their mechanisms, applications in crop breeding and limitations is briefly introduced to provide fundamental understanding and guidance for future utilization.
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