Antimicrobial properties of chlorophyll and hemin incorporated into the polymeric matrix of poly-N-vinylpyrrolidone

Abstract

The increase in the number of antibiotic-resistant strains of microorganisms is becoming more widespread. Metalloporphyrins are promising and modern antimicrobial agents. The most well-known representatives of metalloporphyrins are chlorophyll (Chl) and hemin. This paper presents the results of studies on the effectiveness of Chl and hemin complexes with poly-N -vinylpyrrolidone (PVP) as an antimicrobial agent against Staphylococcus aureus and Escherichia coli. The method for preparing polymeric forms of Chl and hemin is presented. The binding constants of these substances to the polymer were calculated, which were 0.5×105 L/mol for Chl and 3.3×104 L/mol for hemin. Experimental data on the release of substances from the polymeric matrix were obtained. It was found that the complete release of Chl from PVP was observed after 13 h, and hemin – after 10 h. The data on the comparative antimicrobial effect of substances in free and polymeric form were obtained in a microbiological test. Further these results can be used in the development of medicines against microbial infections.