Study of immunomodulating effects of Streptococcus pyogenes B-7612 bacteria on cellular and humoral immunity of pets

Abstract

For the first time, a study was conducted of the effect of living bacteria of the Streptococcus pyogenes avirulent strain B-7612, on the cellular and humoral immunity indicators of domestic animals (dogs) with signs of immunodeficiency. Lyophilizates of live bacteria was administered intradermally to dogs according to the scheme of 0,02-0,04 ml. for 25 days with an interval of 5 days. At the beginning of the experiment and for every 5 days, all animals were sampled to determine immunity parameters: the number of formed elements (leukocytes, leukocyte formula), protein, albumin, globulin, phagocytic number, phagocytic index, phagocytic activity, bactericidal activity of blood serum, lysozyme activity of blood serum. Blood was taken using blood collection systems. Streptococcus pyogenes strain B-7612 has been found to stimulate the cellular immunity, resulting in an increase in the total number of leukocytes (granulocytes, monocytes and lymphocytes), activation of neutrophilic phagocytic activity of the blood, with simultaneous growth of phagocytic index and phagocytic number, the activity of humoral factors is activated, which indicates an increase in the natural stability of the immune system. In the blood of experimental animals there were no significant changes in the amount of total protein, albumin and globulins compared with the control.