Induction of morphogenesis in the callus culture of Lavandula angustifolia Mill.

Abstract

The influence of the nutrient medium hormonal composition on the induction of calluso- and morphogenesis in Lavandula angustifolia Mill., as well as the content of some endogenous hormones (indolylacetic acid, cytokinins, abscisic acid) in morphogenic and non-morphogenic calli, were studied. The leaf explants were isolated from obtained in vitro plants of the Stepnaya and Vdala cultivars. In primary calli with a frequency of 5.7-11.6%, buds and shoots were formed on different Murashige and Skoog (MS) culture media supplemented with 6-benzylaminopurine, thidiazuron, kinetin, and gibberellic acid. In the callus of the first passage in both cultivars, the induction of morphogenesis was observed with the greatest frequency (39.5-43.2%) on the MS medium with the addition of 1.0 mg/l 6-benzylaminopurine and 1.0 mg/l kinetin. Morphogenic calli of the Vdala cultivar were characterized by a higher content of cytokinins, but a lower content of indolylacetic and abscisic acids compared with non-morphogenic ones.