The epitopes analysis and construction of recombinant plasmid of fused ESAT-6/Tb10.4 for tuberculosis vaccine development

Author:

Pambudi Sabar,Irsyad Anwar Shafrial,Utomo Doddy Irawan Setyo,Nurhasanah Astutiati,Novianti Titta

Abstract

Mycobacterium tuberculosis (Mtb) is a pathogenic bacteria responsible for tuberculosis (TB), an infectious disease that poses a significant threat in Indonesia. Despite extensive and thorough research efforts throughout the years, Bacille Calmette-Guerin (BCG) remains the sole authorized vaccination with varying levels of effectiveness. It offers immunity against tuberculosis in children but is not efficacious in treating tuberculosis in adults. Epidemiological modelling indicates that, despite advancements in pharmacological treatments for tuberculosis, the World Health Organization’s efforts to contain the spread of the illness necessitate the development of a novel vaccine with the ability to prevent tuberculosis. The B-cell epitope prediction algorithms have significant medical and economic value because of their practical use in vaccine development. In this study, we employed immunoinformatic prediction tools such as Alphafold, Ellipro, VaxiJen, and IFNepitop to analyze the epitopes of fuse antigen ESAT-6/Tb10.4. We then performed molecular cloning of fuse gene ESAT-6/Tb10.4 into bacteria expression vector pET21d(+). The predicted template modelling (pTM) from Alphafold 3 of our fused protein is 0.57. Which means it might be similar to the true structure. The B cell epitope from Ellipro analysis showed 5 linear and 5 discontinuous epitopes. Our analysis using IFNepitop predicted 126 candidates induced interferon gamma-inducing epitopes out of 198 peptides. Moreover, we successfully fused the ESAT-6 gene and Tb10.4 gene into expression vector pET21d(+) and confirmed by restriction enzyme digestion.

Publisher

EDP Sciences

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3