Author:
Inozemcev P.O.,Grigoriev G.E.,Lepekhova S.A.
Abstract
The study was performed using neonatal livers from Wistar rats. To obtain cells, it is proposed to use a mixed disaggregation method. A significant increase in the amount of the regulatory peptide on the 5th day was revealed to 46.8 (38.4–50.0) ng/ml, and therefore for lyophilization it is better to use a 5-day cell culture containing the maximum amount of HGF. Optimally selected technological conditions made it possible to preserve biologically active substances secreted by liver cells and obtain a stable form of lyophilized liver cells enriched with hepatocyte growth factor.
Reference19 articles.
1. Acute-on-chronic liver failure: terminology, mechanisms and management
2. Liver transplantation in acute liver failure: Dilemmas and challenges
3. Matveev A.V. Hepatoprotectors. Analysis of international studies on drugs from the group of drugs for the liver (Simferopol: IT "ARIAL", 2013)