Antitumor Response of Anti-B7-H3 CAR-T Cells with Humanized scFv in Solid Tumors

Author:

SI Ke,XU Huantian,YE Zheng,JAFFAR ALI Doulathunnisa,HE Cong,DING Bo,YUAN Shubin,DAI Zhu,LI Zhanping,SUN Bo,SHEN Yang,XIAO Zhongdang

Abstract

We prepared a humanized alternative B7-H3 CAR (B7-H3-haCAR-T) targeting B7-H3 which contained the humanized singl-chain varinlde fragment (scFv) from humanized 8H9 Monoclonal Antibody (hu8H9mAb). The antitumor effects of B7-H3-haCAR-T cells were evaluated in B7-H3 overexpressed tumor cells (in vitro) and B7-H3 xenograft models (in vivo). The specific tumour killing ability of B7-H3-haCAR-T in overexpressing B7-H3 tumour cells was verified by cytotoxicity and ELISA tests. In addition, B7-H3 haCAR-T cells were also noted to suppress the tumour growth remarkably well in the xenograft murine models, and the survival time in the haCAR-T cell treatment group was appreciably longer than that in the control group. The specific recognition and highly efficient tumoricidal behavior of B7-H3 haCAR-T provide a basis for future clinical studies with humanized scFv-transduced CAR-T cells targeting solid tumors.

Publisher

EDP Sciences

Subject

Multidisciplinary

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