EFFICIENCY OF USING GENODIAGNOSTIC TEST IN ASSESSMENT OF PERINATAL INFECTION OF YOUNG ANIMALS IN PREVENTION OF LEUKEMIA OF CATTLE

Abstract

This article presents the results of determining the level of perinatal infection of calves with the bovine leukemia virus at the age from birth to colostrum intake and 1-1.5 months after birth when drinking milk from healthy individuals, excluding the alimentary transmission route, with an infection frequency of 5.4% in the surveyed farm. Improvement of molecular diagnostics based on genomic analysis in the scheme of complex antiepizootic/recreational measures for bovine leukemia has been carried out in order to improve the quality of dairy products. As part of the implementation and improvement of the scheme of preventive measures, a positive trend was found, expressed in a tendency to a 3.6-fold decrease in the frequency of detection of BLV infection of young animals. In the course of work, the efficiency of the use of a gene diagnostic test - multiplex PCR-RT – as a tool for the specific prevention of bovine leukemia of calves in the early stages of the disease was shown: the relative diagnostic sensitivity of the PCR-RT technique is at a level not lower than that of the serological method and differs in the side of overestimation by 3.8 times.