Türkiye’nin kuzeybatısındaki bir plantasyonda yerli ve egzotik çam türlerinin ibrelerinde görülen fungal endofitler-Reference-Cited by-同舟云学术

Türkiye’nin kuzeybatısındaki bir plantasyonda yerli ve egzotik çam türlerinin ibrelerinde görülen fungal endofitler

Published:2023-09-15 Issue: Volume: Page:208-212
ISSN:2149-3898
Container-title:Turkish Journal of Forestry | Türkiye Ormancılık Dergisi
language:en
Short-container-title:

Author:

ADAY KAYA Ayse Gülden¹^ORCID,DOĞMUŞ LEHTİJARVİ Tuğba²^ORCID,LEHTİJARVİ Asko Tapio³^ORCID

Affiliation:

1. ISPARTA UNIVERSITY OF APPLIED SCIENCES, YENİŞARBADEMLİ VOCATIONAL SCHOOL, DEPARTMENT OF FORESTRY

2. ISPARTA UNIVERSITY OF APPLIED SCIENCES, FACULTY OF FOREST, DEPARTMENT OF FORESTRY ENGINEERING, FOREST ENGINEERING PR.

3. ISPARTA UNIVERSITY OF APPLIED SCIENCES, SUTCULER PROF.DR.HASAN GURBUZ VOCATIONAL SCHOOL

Abstract

The endophytic fungi present in needles of Pinus sylvestris, Pinus pinaster, Pinus nigra, Pinus taeda and Pinus radiata were investigated in Kerpe Research Forest, İzmit in 2016. Ten trees of each pine species were sampled systematically. Previous years green needles were sampled from the lower part of the canopy, from two equally spaced positions around the tree. Each needle was surface sterilized and cut into 0.5 cm sections then individually placed onto malt extract agar plates. DNA was ex-tracted from reprensentative seven isolates and amplified using primers ITS1 and ITS4 targeting the nuclear 5.8S rDNA gene and the two ITS regions flanked between 18S and 28S rDNA genes. Amplicons sequenced in both directions using the univer-sal fungal primers ITS1 and ITS4. Isolations from a total of 1000 needles (200 from each pine species) yielded 750 fungal isolates. Pestalotiopsis funerea (Desm.) Steyaert was isolated at the highest frequency followed by Acremonium sp., Cladio-sporum sp. and Cyclaneusma minus.

Publisher

Turkish Journal of Forestry

Subject

Building and Construction

Reference38 articles.

1. Agrios, G.N., 1988. Plant Pathology. Academic Press, New York, USA.

2. Arnold, A.E., Henk, D.A., Eells, R.L., Lutzoni, F., Vilgalys, R., 2007. Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and envi-ronmental PCR. Mycologia, 99: 185-206.

3. Bills, G.F., 1996. Isolation and analysis of endophytic fungal communities from woody plants. See Reference, 124: 31–65.

4. Brown, K.B., Hyde, K.D., Guest, D.I., 1998. Preliminary studies on endophytic fungal communities of Musa acuminata spe-cies complex in Hong Kong and Australia. Fungal Diversity, 1: 27-51.

5. Botella, L., Dize, J.J., 2011. Phylogenic diversity of fungal endo-phytes in Spanish stands of Pinus halepensis. Fungal Diversity, 47:9-18.