Response Formation of Cattleya Orchid Leaf Callus (<i>Cattleya</i> Sp.) with the Addition of <i>2.4 Dichlorophenoxy Acetic Acid </i>and <i>6-Benzyl Amino Purine In Vitro</i>

Abstract

Orchid Cattleya sp. has a very high commercial value in the market both locally and internationally. Although the price of the orchid tends to be expensive, the value it has is much more expensive. So cattleya is always a top priority for hobbyists and collectors [1]. Cattleya is one of the orchid genera that have flowers with large sizes and is known as "The Queen of Orchids" because it has the beauty of color variations in its flowers [2]. In addition to having beauty in its flowers, bright flower color, and fragrant smell, Cattleya Orchid in general has a large flower size compared to other orchid flowers [3]. This study aimed to determine the best combination for callus growth from the orchid leaf explant Cattleya sp. with the combination of media using the ingredients MS + 6-Benzyl Amino Purine (0; 0.3; 0.6 ppm) and 2.4 Dechlorophenoxy Acetic Acid (0; 1; 2; 3 ppm) with three repetitions for each treatment. The data from the study showed that the best concentration to spur the growth of the callus of the explanatory orchid leaves of Cattleya sp. At the treatment of 2.4-D 1 ppm + BAP 0.6 ppm with a percentage of callus formed about 60% with a green callus color and compact callus texture and medium callus growth (++).