Detection of Cancer Cells Using G-Rich DNA Based Target Binding-Switching Calorimetric Biosensor-Reference-Cited by-同舟云学术

Detection of Cancer Cells Using G-Rich DNA Based Target Binding-Switching Calorimetric Biosensor

Published:2022-08-29 Issue: Volume: Page:
ISSN:1662-0356
Container-title:Advances in Science and Technology
language:
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Author:

Babakuliyev Alisir¹,Maiti Niladri¹,Antony Annie Aglin²,Ansari Mohammad Javed³,Chobe Santosh S.⁴,Dixit Chandra Kumar⁵

Affiliation:

1. Tishk International University

2. Mepco Schlenk Engineering College

3. Prince Sattam Bin Abdul Aziz University

4. M.G.V's L.V.H Arts, Science and Commerce College

5. Dr. Shakuntala Misra National Rehabilitation University

Abstract

This paper reports the G-rich ssDNA for the colorimetric detection of cancer cells. The ssDNA-1 sequence has explored for the potential application of “Turn-On” colorimetric sensor for selective and sensitive detection of cancer cells. While complementary G-rich DNA strand form G-quadruplex with hemin molecule, which is more effective to catalyze the peroxidase mimicking activity towards TMB chromogenic substrate. The ssDNA-1 exhibits good selectivity for cancer cells. The colorimetric intensity of TMB was enhanced upon interaction of leukemic lymphoblasts cancer cells. The effect of pH has turned the selective sensing performances of the biosensor for detecting cancer cells with a lower detection limit of 0.54 nM, 0.18 nM, and 0.2 nM respectively.

Publisher

Trans Tech Publications Ltd

Link

https://www.scientific.net/AST.117.73.pdf

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