Identification of Specific DNA Fragments in the Superior Mutant Plant of Rodent Tuber Accession Pekalongan (<i>Typhonium flagelliforme</i>) Based on Sequencing Analysis

Abstract

Cancer is a large group of diseases that occur in almost any organ or tissue of the body when abnormal cells get out of normal and invade adjacent cells. In women especially, breast cancer is among the most common types of cancer. These cancer cells will form a tumor that can be seen on an x-ray. Some traditional breast cancer treatments have been carried out, including using local herbal plants such as rodent tubers (Typhonium flagelliforme). Rodent tuber contains stigmasterol which has a high anticancer effect and was found in several mutant clones. The purpose of this study was to analyze DNA differences between mutant and wild-type rodent tuber plants based on specific RAPD markers and sequence differences in each sample. The RAPD OPB18 primer was successfully amplifies polymorphic DNA bands with sizes ranging from 700 to 1000 bp. As a result of the sequencing analysis, separate segments in wild-type and mutant plants had different sequences. In comparing the test sequence to the NCBI database, it was found that wild-type rodent tuber samples matched Gossypium raimondii (81.82%) and BLAC (bulk highest anticancer compound) rodent tuber mutant plants has similarity with Slanum pinnasectum about 87.36%. Based on this results, the similarity of rodent tuber sequences is possible to be developed in the specific sequences that encode a gene, especially genes encoding anticancer compounds.