Affiliation:
1. Pavol Jozef Safarik University in Kosice
2. Pavol Jozef Safarik Univeristy
3. Slovak Academy of Sciences
Abstract
Magnetic-bead separation or purification serves as a technique for effective isolation of biomolecules. In presented work we prepared and characterized core-shell magnetic nanoparticle samples consisted of Fe3O4 core coated with SiO2 shell. Samples were subsequently coated with ligands MPTMS (3-(mercaptopropyl)trimethoxysilane), CPTMS (3-(chloropropyl)trimethoxysilane) and MMSP (3-(trimethoxysilyl)propyl methacrylate) with aim to increase the number of active centers for specific binding with RNA. Such samples were further investigated for their magnetic properties, size, and morphology. Magnetic properties were studied in DC field up to 5 T in temperature range 5 – 300 K. Size and morphology were determined from SEM micrographs and elemental compositions of the samples were investigated using EDX analysis. Modification of nanoparticle surface with different ligands leads to modification of active centers on the SiO2 surface on which the DNA and RNA molecules can be bounded. It also causes the change in magnetic and structural properties of nanoparticles.
Publisher
Trans Tech Publications, Ltd.
Reference8 articles.
1. Functional magnetic particles for medical application;Shinkai;Journal of Bioscience and Bioengineering
2. Magnetic separation techniques in diagnostic microbiology;Olsvik;Clinical Microbiology Reviews
3. Magnetism of Nanoparticles: Effect of the Organic Coating;Abdolrahimi;Nanomaterials,2021
4. Functionalization of Magnetic Nanoparticles with Organic Ligands toward Biomedical Applications;Yang;Advanced NanoBiomed Research,2021
5. M. Benelmekki & Ll. M. Martinez "Magnetophoresis of iron oxide nanoparticles: A tool for synthesis monitoring and biomagnetic applications" "Drug Delivery and Nanomedicine" vol 5. Editor J.N. Govil, Studium Press LLC, USA (2013).