Affiliation:
1. King Mongkut’s University of Technology North Bangkok
Abstract
Materials modification with surface functional group for bioactive film or sensor surface is important in many applications. A suitable surface for each system can be shown high efficiency of detection. In this work, two different surfaces via covalent binding are created on gold substrate for finding the suitable surface in medical applications. The liver cancer biomarker, alpha-fetoprotein (AFP) was detected on two surfaces; one is carboxy dextran and another is a modified polymethyl metacrylate (PMMA). The substrates were improved by physical and chemical binding for AFP antibody immobilization. Contact angle and surface Plasmon resonance (SPR) were used to study the characteristic of surfaces. The result was found that the carboxy dextran gave higher detection than PMMA. Moreover, the dextran surface was studied in real serum samples. It was shown the significantly different between positive and negative AFP serum which is preliminary results for testing in more clinical samples further.
Publisher
Trans Tech Publications, Ltd.
Subject
Mechanical Engineering,Mechanics of Materials,General Materials Science
Reference5 articles.
1. Langer, R.; Tirrell, D. A. Designing materials for biology and medicine. Nature Vol 248,(2004),p.487−492.
2. Chu, P. K.; Chen, J. Y.; Wang, L. P.; Huang, N. Plasma-surface modification of biomaterials. Mater. Sci. Eng., R Vol 36, (2002),p.143−206.
3. B.Wook Park, D.Shik Kim, and D.Young Yoon, Surface modification of gold electrode with gold nanoparticles and mixed self-assembled monolayers for enzyme biosensors', Korean J. Chem. Eng., Vol 8(1), (2011),pp.64-70.
4. Lofas, S. Dextran modified self-assembled monolayer surfaces for use in biointeraction analysis with surface plasmon resonance. Pure & Appl.Chem, Vol 67(1995), pp.829-834.
5. T.Wangkam, Kitti Boonperm, Phatra Khomkrachang,Toemsak Srikhirin, Verayuth Praphanphoj, Boonsong Sutapan , Armote Somboonkaew and Rathasart Amarit, Hepatocellular Carcinoma Biomarker Detection by Surface Plasmon Resonance Sensor, Advanced Materials Research, Vol. 1131 (2016).