Microfluidic Amperometric Detection Sensor with Integrated Ag/AgCl Reference Electrode

Abstract

In this paper, we presented a novel method for fabricating the integrated electrochemical sensor based on a microfluidic device. The device included: printed circuit board (PCB) substrate with vertical embedded electrodes; polymethyl methacrylate (PMMA) with micro structures; PMMA cover with amperometric detection sensor (ADS). The ADS employed three-electrode sensor system which was composed by two Au electrodes and an Ag/AgCl reference electrode. The polyvinyl chloride (PVC) membrane was used as ion-exchange medium of reference electrode and installed between Au electrodes. Then, the potential changes and the standardization oxidation-reduction experiments were executed to characterize the integrated reference electrode performance. Finally, the ADS system was tested by cyclic voltammetry (CV).