Discrimination and Simultaneous Determination of 6-BA and KT by Polarized Synchronous Light Scattering and Synchronous Light Scattering Spectrum

Author:

Zhao Xiao Hui1,Zhu Qian Hua1,Yang Qiong1,He Wei1,Zhou Shang1,Li Xiao Yan1,Yang Ji Dong2

Affiliation:

1. Yangtze Normal University

2. Chong Qing Three Gorges University

Abstract

In pH=5.70 B-R buffer solution, Na2WO4 and rhodamine 6G(R6G) could react with natural cytokinins of 6-benzylaminourine (6-BA) and kinetin (KT) and resulted in a great enhacement of synchronous light scattering (SLS) signals. This article first established the degree of polarized synchronous light scattering (P) based on the measurements of the polarized synchronous light scattering signals to distinguish two natural cytokinins of 6-BA and KT. Simultaneously based on the synchronous scattering spectrum and the double standard curve method, a novel method for simultaneous determination of 6-BA and KT was developed by SLS spectralmethod. The method was applied to simultaneous determination of 6-BA and KT in balsam pear skin and tomato skin with satisfactory results.

Publisher

Trans Tech Publications, Ltd.

Subject

General Engineering

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5. X.Y. Li, S. Zhou, W. He and J.D. Yang: Luminescence, Vol. (2012) No.

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