Study on the Mobilities with Replaceable Linear Polyacrylamide (LPA) Gel Electrophoresis

Author:

Wang Jie1,Guo Li Na1,Wang Li Qiang2,Shi Yan3,Zheng Hua4,Lu Zu Kang2

Affiliation:

1. South China Normal University

2. Zhejiang University

3. China Jiliang University

4. Fujian Normal University

Abstract

The replaceable Linear polyacrylamide(LPA) gel was used as the sieving medium; An extensive series of experiments has been performed using DNA fragments ranging in size from 80 to 587 base pairs with laser induced fluorescence detection(LIFD). The electric field was changed from 100V/cm to 375V/cm. The mobilities with LPA in different electric field strength were studied. Although the LPA sieving matrix is commonly used in biological chemistry, these mobilities have not previously been reported. It was different from with the cross-link polyacrylamide and agar gels. The new modification of Ogston model was initiated. This work complements our systematic study on electrophoretic mobilities under different conditions, and will help to further optimize and improve high performance DNA separation in microfluidic applications.

Publisher

Trans Tech Publications, Ltd.

Subject

General Engineering

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