Made Polypeptide from Sipunculs Nudus by Papain Enzymolysis and its Effects on Hydroxy Radical Cleaning-Reference-Cited by-同舟云学术

Made Polypeptide from Sipunculs Nudus by Papain Enzymolysis and its Effects on Hydroxy Radical Cleaning

Published:2013-05 Issue: Volume:699 Page:360-366
ISSN:1662-8985
Container-title:Advanced Materials Research
language:
Short-container-title:AMR

Author:

Zhu Yin Ling¹,Li Si Dong¹,Liao Yan¹,Li Jiang Ming¹

Affiliation:

1. Guangdong Ocean University

Abstract

The polypeptide of Sipunculs nudus was prepared by papain. The optimum papain enzymolysis process to Sipunculs nudus was confirmed by means of single factor analysis and orthogonal test, it showed as below: 300U/g of enzyme addition amount, 50°C of temperature, 60min of enzymolysis time and pH7.0. The clearance rate of hydroxy radical is 95.42% according to the optimum process on Sipunculs nudus enzymolysis. The HPLC chromatogram showed that the prepared component of antioxidative active polypeptide according to such condition is single and its molecular weight is 5868. The results of experiment shows that Sipunculs nudus polypeptide have high inoxidizability and high removing free radicals ability.

Publisher

Trans Tech Publications, Ltd.

Subject

General Engineering

Link

https://www.scientific.net/AMR.699.360.pdf

Reference10 articles.

1. Chao-Hua ZHANG, Takeshi Suzuki, Yum ikoYoshie. Study on Tasty Substance and Functional Components of the Dry Sha-chong (Sipunculus nudus) [J]. Journal of Zhanjiang Ocean University, vol. 20, no. 2, 2000 , pp: 24-27.

2. Fjita H, ashikawa M. A product-type-ACE-inhibitorypeptide derived from fish protein[J]. Immunophamacplogy, 1999, 44(1-2): 123-127.

3. Jun] Li, Zhi-Shen Jia. The Progress of the Research on the Antioxidants from Marine Living Things. vol. 12, no. 4, 1999, pp: 92-96.

4. Dan-Qing LEI, Xian-Guo ZHOU, Hui ZHANG, etc. Study on the Antioxidant Activities of Enzymatic Hydrolysa from Oyster. Lishizhen Medicine and Materia Medica, vol. 19, no. 11, 2008, pp: 2674-2676.

5. The Basis of Biochemistry Experiment(the second edition)[M] . Beijing: Higher Education Press, 1999: 177-180.

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Bioactive Properties of Peptides and Polysaccharides Derived from Peanut Worms: A Review;Marine Drugs;2021-12-22