Characterization and Functional Expression of Xylose Isomerase from <i>Thermus thermophilus</i>-Reference-Cited by-同舟云学术

Characterization and Functional Expression of Xylose Isomerase from Thermus thermophilus

Published:2013-01 Issue: Volume:641-642 Page:919-922
ISSN:1662-8985
Container-title:Advanced Materials Research
language:
Short-container-title:AMR

Author:

Lu An Gen¹,Yang Ze Xi²,Wang Fei²,Xu Lang²,Deng Wen Ying²,Meng Jian Zong¹,Huang Zi Qi¹,Qi Xiang Hui¹

Affiliation:

1. Guangxi Zhuang Autonomous Region Center for Analysis and Test Research

2. Jiangsu University

Abstract

Ethanol produced from hexose and pentose sugars hydrolysated by lignocellulose is an environment-friendly alternative to fossil fuels. Xylose isomerase is the major rate-limiting enzyme in the ethanol synthesis biologically pathway of xylose fermentation. In present study, xylA gene encoding xylose isomerase was cloned from Thermus thermophilus and overexpressed in E. coli BL21. Purified recombinant enzyme was used to study the enzymatic characterization. Specific activity of recombinant PDOR was 19.6 U/mg. Optimal temperature and pH were 80 °C, 8.0, respectively. Km and Vmax values were 15.9 mM, 22.8 U/mg. This research may form a basis for the future application of xylose isomerase.

Publisher

Trans Tech Publications, Ltd.

Subject

General Engineering

Link

https://www.scientific.net/AMR.641-642.919.pdf

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