The Effect of Electrode’s Material on Immobilization of Sulfite Oxidase Enzyme in Construction of Sulfite Biosensors

Abstract

Electrodes have an important role in performance of sulfite detector biosensors. To increase efficiency of performance, using immobilizing processes in the structure of an enzyme electrode is very important. Enzymes can be connected to the working electrode by crosslinking and trapping methods. In this paper, sulfite oxidase enzyme has immobilized on aluminum, screen-printed carbon and glassy carbon electrodes in different ways. Flow production measurement of amperometric cycle showed that aluminum and screen-printed carbon electrode had better performance at concentrations less than 110 micro-molar, but in higher concentrations, glassy carbon electrode showed highest conductivity.