Effect of Simultaneous Saccharification and Fermentation (SSF) Time on Ethanol Production from Spent Medium of Oyster Mushroom (Pleurotus ostreatus)

Abstract

Bioethanol is considered as the most promising prospective renewable energy source. One of the most potential lignocellulose material for bioethanol feedstock is spent media (SM) of edible mushroom. Pleurotus ostreatus is more selective to degrade lignin than holocellulose component, therefore the SM is very compatible as a bioethanol feedstock. This study was observed the influence of variation of cultivation time of oyster mushroom (P.ostreatus) into the SM chemical content and its ethanol production yield by using simultaneous saccharification and fermentation method. The results showed that the difference of cultivation time did not show the significant result on SM chemical content, except the hot water soluble extractive content. The highest hot water soluble extractive content was found in SM with 110 days of cultivation time (27.68%). The highest hydrolysis rate was found at 90 days of cultivation time (15.65%) and 48 and 72 hours saccharification time (14.77% and 14.78%). The highest reducing sugar content was found at 110 days of cultivation time (4.89 g/L). The highest ethanol content was found in SM with a combination of 90 days cultivation time and 48 hours saccharification time (1.696 g/L). The 90 days cultivation time was enough to produce SM that can be used as raw material for bio-ethanol production.