Effect of Sm-Au on Silver Staining Results and it ́s UV-Vis Absorption Spectrum

Abstract

Sm particles and gold nanoparticles were prepared respectively. Effects of Sm-Au particles on silver staining results were studied, and UV-Vis absorption spectrum of Sm-Au particles solution was studied. Time and color of sport with Sm-Au particles is longer and darker than that of with gold nanoparticles. The time of sport with Sm-Au particles is as long as 28.5 min, which is 2.6 times as long as with gold nanoparticles. Although amount of gold nanoparticles reduced 80%, the color of sport with Sm-Au particles is darker than that of with gold nanoparticles. In 200.00-800.00 nm, Sm particles solution and gold nanoparticles solution has one absorption peak, respectively, and λmaxis 276 nm, 521 nm respectively. Sm-Au particles solution has two absorption peaks, λmax (Sm)and λmax (Au)are 276 nm and 523 nm respectively. λmaxof Sm particles is constant, and λmaxof Au nanoparticles occurs red moving. Sm particles and gold nanoparticles may have interaction.