Applied Technology in Using HPLC for Determining Aflatoxin Contents in Peanuts-Reference-Cited by-同舟云学术

Applied Technology in Using HPLC for Determining Aflatoxin Contents in Peanuts

Published:2014-08 Issue: Volume:1022 Page:76-80
ISSN:1662-8985
Container-title:Advanced Materials Research
language:
Short-container-title:AMR

Author:

Shi Yong Gang¹,Zhong Wei¹,Wen Hao¹,Ma Ju¹,Sun Ping²

Affiliation:

1. Logistical Engineering University

2. Chongqing Nonferrous Metal Institute

Abstract

Aflatoxins in grinded peanuts were extracted with 70% methanol and purified with immuno affinity column. In order to enhance the test sensitivity, the Aflatoxin was derived by trifluoroacetic acid. the derived Aflatoxin B1, B2, G1 and G2 were analyzed by HPLC with fluorescence detector. The determination could be completed in 14 minutes. The detect limits of Aflatoxin B1, B2, G1 and G2 were 0.04μg/kg, 0.01μg/kg, 0.20μg/kg and 0.02μg/kg respectively, the recoveries were from 81.0% to 115.0%, the relative standard deviations were less than 8.2%.

Publisher

Trans Tech Publications, Ltd.

Subject

General Engineering

Link

https://www.scientific.net/AMR.1022.76.pdf

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