Affiliation:
1. Shanghai Ocean University
2. Shanghai Ocean Aquarium
Abstract
To find out the etiology and prevention methods of the death ofCaesio diagramma. Isolate the pathogen from the fester surface, liver, kidney , intestinal and other organizations of the diseasedCaesio diagramma; After the purification of the predominant strains, the phylogenetic analysis of genetic relationships was done by determining 16s rDNA gene and Homology comparison was done with the BLAST sequence detected and registered in GenBank sequence ; In the end, phylogenetic trees were constructed and the drug sensitivity experiment and pathological observation were taken.Aeromonas hydrophilais the cause of the death ofCaesio diagramma, it is highly sensitive to chloramphenicol, florfenicol, cefotaxime, gentamicin, amikacin; The pathological results indicate that the wax buildups causes increasing energe consumption ofCaesio diagramma,which decreased the resistance ofCaesio diagrammaand even leads to secondary bacterial infection. Pathogen infected the liver and spleen tissues which causes the disease and death ofCaesio diagramma.Aeromonas hydrophilacauses the death ofCaesio diagramma, and the results have an important guiding significance for the healthy breeding ofCaesio diagrammaand other ornamental fish
Publisher
Trans Tech Publications, Ltd.
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